Plasmid

Part:BBa_I713006:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-25)


T7BLUE T7X


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1304
    Illegal SapI site found at 221


Design Notes

Do not express T7 RNAP gene. However, it is reported that CTG can also initiate the translation.


Source

BBa_I713007 BBa_I713003

References